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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NIPSNAP3A Polyclonal antibody

NIPSNAP3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10751-1-AP

Synonyms

TassC, Target for Salmonella secreted protein C, Protein NipSnap homolog 4, Protein NipSnap homolog 3A, NipSnap4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    WB analysis using 10751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    WB analysis of COLO 320 using 10751-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    WB analysis of HEK-293 using 10751-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IP experiment of mouse brain using 10751-1-AP

    IP result of anti-NIPSNAP3A (IP:10751-1-AP, 4ug; Detection:10751-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IF Staining of HEK-293 using 10751-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NIPSNAP3A antibody (10751-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10751-1-AP targets NIPSNAP3A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIPSNAP3A fusion protein Ag1143 相同性解析による交差性が予測される生物種
Full Name nipsnap homolog 3A (C. elegans)
Calculated molecular weight 28 kDa
Observed molecular weight 28-33 kDa
GenBank accession numberBC005935
Gene symbol NIPSNAP3A
Gene ID (NCBI) 25934
RRIDAB_2298420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IHC protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IF protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IP protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

NIPSNAP3A regulates cellular homeostasis by modulating mitochondrial dynamics

Authors - Run Yan
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kristina (Verified Customer) (01-20-2022)

I tried a few antibodies for NIPSNAP3A in the past and this one was the most precise with the best result so far.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: LUHMES
NIPSNAP3A Antibody Western Blot validation (1:1000 dilution) in LUHMES (Cat no:10751-1-AP)