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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NISCH Polyclonal antibody

NISCH Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 13813-1-AP

Synonyms

I1R, I-1 receptor candidate protein, I-1, I 1 receptor candidate protein, I 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NISCH Polyclonal antibody (13813-1-AP)

    WB analysis using 13813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13813-1-AP (NISCH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NISCH Polyclonal antibody (13813-1-AP)

    IP experiment of Jurkat using 13813-1-AP

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with Jurkat cells lysate 1560 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NISCH Polyclonal antibody (13813-1-AP)

    IP experiment of mouse brain using 13813-1-AP

    IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NISCH Polyclonal antibody (13813-1-AP)

    IHC staining of human stomach using 13813-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13813-1-AP (NISCH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13813-1-AP targets NISCH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NISCH fusion protein Ag4880 相同性解析による交差性が予測される生物種
Full Name nischarin
Calculated molecular weight 1504 aa, 167 kDa
Observed molecular weight 190-200 kDa
GenBank accession numberBC038102
Gene symbol NISCH
Gene ID (NCBI) 11188
RRIDAB_10693526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NISCH antibody 13813-1-APDownload protocol
IHC protocol for NISCH antibody 13813-1-APDownload protocol
IP protocol for NISCH antibody 13813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Anesth Analg

Dexmedetomidine Ameliorates Perioperative Neurocognitive Disorders by Suppressing Monocyte-Derived Macrophages in Mice With Preexisting Traumatic Brain Injury.

Authors - Natsumi Kii
View Article
humanWB,IF

Biochem Biophys Res Commun

Expression of Nischarin negatively correlates with estrogen receptor and alters apoptosis, migration and invasion in human breast cancer.

Authors - Chan Chang
View Article