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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NKAP Polyclonal antibody

NKAP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27437-1-AP

Synonyms

NF kappa B activating protein, NFKB activating protein, NKAP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NKAP Polyclonal antibody (27437-1-AP)

    WB analysis using 27437-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27437-1-AP (NKAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27437-1-AP targets NKAP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKAP fusion protein Ag26637 相同性解析による交差性が予測される生物種
Full Name NFKB activating protein
Calculated molecular weight 415 aa, 47 kDa
Observed molecular weight47-50 kDa
GenBank accession numberBC015354
Gene symbol NKAP
Gene ID (NCBI) 79576
RRIDAB_3085959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NKAP is a multifunctional nuclear protein that is involved in the activation of the ubiquitous transcription factor NF-kappaB. NKAP is associated with the the histone deacetylase HDAC3 and with the Notch corepressor complex, and it thereby acts as a transcriptional repressor of Notch target genes. It is also required for alphabeta T cell development. A related pseudogene has been identified on chromosome X, while a related and intronless retrocopy, which has an intact CDS and may be functional, is located on chromosome 6.

Protocols

Product Specific Protocols
WB protocol for NKAP antibody 27437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols