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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of mouse colon using 16699-1-AP (same clone as 16699-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NKD2 Polyclonal antibody (16699-1-AP)

    WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NKD2 Polyclonal antibody, PBS Only

NKD2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16699-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Naked-2, Naked2, Naked 2, hNkd2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16699-1-PBS targets NKD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKD2 fusion protein Ag10094 相同性解析による交差性が予測される生物種
Full Name naked cuticle homolog 2 (Drosophila)
Calculated molecular weight 50 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC012176
Gene Symbol NKD2
Gene ID (NCBI) 85409
RRIDAB_2151468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969F2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).