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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NKIRAS2 Polyclonal antibody

NKIRAS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10174-1-AP

Synonyms

KBRAS2, KappaB-Ras2, kappaB Ras2, Kappa B-Ras protein 2, Kappa B Ras protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NKIRAS2 Polyclonal antibody (10174-1-AP)

    WB analysis of HEK-293 using 10174-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NKIRAS2 Polyclonal antibody (10174-1-AP)

    WB analysis of HepG2 using 10174-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NKIRAS2 Polyclonal antibody (10174-1-AP)

    IP experiment of HEK-293 using 10174-1-AP

    IP result of anti-NKIRAS2 (IP:10174-1-AP, 4ug; Detection:10174-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NKIRAS2 Polyclonal antibody (10174-1-AP)

    IF Staining of A431 using 10174-1-AP

    Immunofluorescent analysis of A431 cells using 10174-1-AP (NKIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10174-1-AP targets NKIRAS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKIRAS2 fusion protein Ag0221 相同性解析による交差性が予測される生物種
Full Name NFKB inhibitor interacting Ras-like 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC007450
Gene symbol NKIRAS2
Gene ID (NCBI) 28511
RRIDAB_2298444
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.

Protocols

Product Specific Protocols
WB protocol for NKIRAS2 antibody 10174-1-APDownload protocol
IF protocol for NKIRAS2 antibody 10174-1-APDownload protocol
IP protocol for NKIRAS2 antibody 10174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis.

Authors - Stephanie Beel
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Andrea (Verified Customer) (09-18-2020)

The antibody works great in Western Blot. Good signal and specificity, which was tested on knockout cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: pancreas, different human and mouse cell lines