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  • Featured Product
  • KD/KO Validated

NKRF Monoclonal antibody, PBS Only

NKRF Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H10C8

Cat no : 68230-1-PBS

Synonyms

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NKRF Monoclonal antibody (68230-1-Ig)

    WB analysis of HEK-293 using 68230-1-Ig (same clone as 68230-1-PBS)

    WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NKRF Monoclonal antibody (68230-1-Ig)

    WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NKRF Monoclonal antibody (68230-1-Ig)

    WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68230-1-PBS targets NKRF in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NKRF fusion protein Ag6374 相同性解析による交差性が予測される生物種
Full Name NFKB repressing factor
Calculated molecular weight 78 kDa
Observed molecular weight100 kDa
GenBank accession numberBC068514
Gene symbol NKRF
Gene ID (NCBI) 55922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.