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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTF1; NKX2-1 Polyclonal antibody

TTF1; NKX2-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21777-1-AP

Synonyms

TTF1, NKX2 1, NK2 homeobox 1, NK 2, Homeobox protein Nkx-2.1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of HeLa using 21777-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of HeLa using 21777-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of A549 using 21777-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of mouse lung using 21777-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of mouse brain using 21777-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using TTF1; NKX2-1 Polyclonal antibody (21777-1-AP)

    WB analysis of rat brain using 21777-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat brain tissue, A549 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21777-1-AP targets TTF1; NKX2-1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name NK2 homeobox 1
Calculated molecular weight 42 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_003317
Gene symbol TTF1
Gene ID (NCBI) 7080
RRIDAB_2878919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.

Protocols

Product Specific Protocols
WB protocol for TTF1; NKX2-1 antibody 21777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol Pharmacol

Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation

Authors - Qi Xu
View Article