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- KD/KO Validated
NKX2-5 Polyclonal antibody
NKX2-5 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 13921-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | human brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IF | See 3 publications below |
Product Information
13921-1-AP targets NKX2-5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NKX2-5 fusion protein Ag4930 相同性解析による交差性が予測される生物種 |
| Full Name | NK2 transcription factor related, locus 5 (Drosophila) |
| Calculated molecular weight | 324 aa, 35 kDa |
| Observed molecular weight | 30-42 kda |
| GenBank accession number | BC025711 |
| Gene symbol | NKX2-5 |
| Gene ID (NCBI) | 1482 |
| RRID | AB_1851479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| IHC protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| IF protocol for NKX2-5 antibody 13921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells. | ||
Am J Physiol Heart Circ Physiol Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5. | ||
J Appl Toxicol The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice. | ||
Mol Med Rep H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes. | ||
Int J Clin Exp Pathol SDF-1α promotes repair of myocardial ischemic necrosis zones in rats. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Rebecca (Verified Customer) (10-25-2022) | 20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.
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