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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NKX3-1 Polyclonal antibody, PBS Only

NKX3-1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13069-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using NKX3-1 Polyclonal antibody (13069-1-AP)

    WB analysis of LNCaP using 13069-1-AP (same clone as 13069-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using NKX3-1 Polyclonal antibody (13069-1-AP)

    WB analysis of human testis using 13069-1-AP (same clone as 13069-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 13069-1-AP (NKX3-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NKX3-1 Polyclonal antibody (13069-1-AP)

    IHC staining of human prostate cancer using 13069-1-AP (same clone as 13069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NKX3-1 Polyclonal antibody (13069-1-AP)

    IHC staining of human prostate cancer using 13069-1-AP (same clone as 13069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13069-1-AP (NKX3-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13069-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13069-1-PBS targets NKX3-1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKX3-1 fusion protein Ag4070 相同性解析による交差性が予測される生物種
Full Name NK3 homeobox 1
Calculated molecular weight 234 aa, 26 kDa
Observed molecular weight34-38 kDa
GenBank accession numberBC074864
Gene symbol NKX3-1
Gene ID (NCBI) 4824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NKX3-1, also named as NKX3.1 and NKX3A, belongs to the NK-3 homeobox family. It is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. NKX3-1 plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells(PMID:19462257). The calcualted molecular weight of NKX3-1 is 26 kDa, but modified NKX3-1 is about 32-35 kDa. (PMID: 18597829)