Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20974-1-AP targets NKX6-1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | NK6 homeobox 1 |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 45-52 kDa |
| GenBank accession number | NM_006168 |
| Gene Symbol | NKX6-1 |
| Gene ID (NCBI) | 4825 |
| RRID | AB_2918068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78426 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NK6 homeobox 1 (NKX6-1) is a potent bifunctional transcription regulator that binds to AT-rich DNA sequences within the promoter region of target genes. NKX6-1 belongs to the class II proteins of neuronal progenitor factors, and the function of it is to enhance proliferation, glucose-stimulated insulin secretion, and survival in mature pancreatic beta-cells, and it is also essential in regional patterning and neuronal fate determination in central nervous system.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NKX6-1 antibody 20974-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |