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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse splenocytes using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    WB analysis of mouse splenocytes using 84416-5-RR (same clone as 84416-5-PBS)

    mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    IHC staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    IHC staining of rat spleen using 84416-5-RR (same clone as 84416-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat thymus tissue using NKp46/NCR1 Recombinant antibody (84416-5-RR)

    IHC staining of rat thymus using 84416-5-RR (same clone as 84416-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84416-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84416-5-RR

    Affinity and Kinetic Characterization of 84416-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.

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Rat NKp46/NCR1 Recombinant antibody, PBS Only

NKp46/NCR1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84416-5-PBS
Clone No.241811H7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Ly94, Kilr1, CD335, Ar1, Activating receptor 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84416-5-PBS targets NKp46/NCR1 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name natural cytotoxicity triggering receptor 1
Calculated molecular weight37kDa
Observed molecular weight50-60 kDa
GenBank accession numberNM_057199.1
Gene Symbol NKp46/NCR1
Gene ID (NCBI) 117547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Z0H5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).