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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NK-92 cells using NKp46/NCR1 Recombinant antibody (85021-4-RR)

    IF Staining of NK-92 using 85021-4-RR

    Immunofluorescent analysis of (4% PFA) fixed NK-92 cells using NKp46/NCR1/CD335 antibody (85021-4-RR, Clone: 242549C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NKp46/NCR1 Recombinant antibody

NKp46/NCR1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 85021-4-RR
Clone No.242549C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

NCR 1, Natural killer cell p46-related protein, LY94, hNKp46, CD335

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inNK-92 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85021-4-RR targets NKp46/NCR1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name natural cytotoxicity triggering receptor 1
Calculated molecular weight34kDa
Gene Symbol NCR1
Gene ID (NCBI) 9437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76036-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NKp46, also known as natural cytotoxicity triggering receptor 1 (NCR1), is a type I transmembrane protein belonging to the immunoglobulin superfamily. NKp46 is a key activating receptor expressed on natural killer (NK) cells. It plays a key role in the innate immune response by recognizing and binding to viral hemagglutinins and other ligands on infected or tumor cells. NKp46 mediates cytotoxicity and cytokine production upon engagement with its ligands, contributing to the elimination of infected or malignant cells. Its expression and function are tightly regulated, and dysregulation of NKp46 has been implicated in various diseases, including viral infections, cancer, and autoimmune disorders. (PMID: 11234016; 29329948; 9730896; 9314561).

Protocols

Product Specific Protocols
IF protocol for NKp46/NCR1 antibody 85021-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols