Validation Data Gallery
Tested Applications
Positive WB detected in | Daudi cells, Jurkat cells, Raji cells, Ramos cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30455-1-AP targets NLRC3 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NLRC3 fusion protein Ag32323 相同性解析による交差性が予測される生物種 |
Full Name | NLR family, CARD domain containing 3 |
Calculated molecular weight | 115 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | NM_178844 |
Gene Symbol | NLRC3 |
Gene ID (NCBI) | 197358 |
RRID | AB_3086322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q7RTR2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NLRC3 is a member of the pattern recognition receptors nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) family, and plays a pivotal regulatory role in modulating the activation of immune cells. In macrophages, NLRC3 inhibits the activation of the NF-κB signaling pathway, the STING/TBK1 signaling pathway, and the formation of the inflammasome. In the context of T cells immune response, NLRC3 prevents the activation of T cells by regulating the function of dendritic cells and directly influencing the function of T cells.
Protocols
Product Specific Protocols | |
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WB protocol for NLRC3 antibody 30455-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |