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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NLRP10 Recombinant antibody, PBS Only

NLRP10 Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230402C1

Cat no : 83131-8-PBS

Synonyms

Nucleotide-binding oligomerization domain protein 8, NOD8, NALP10, NACHT, LRR and PYD domains-containing protein 10, CLR11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using NLRP10 Recombinant antibody (83131-8-RR)

    WB analysis of mouse heart using 83131-8-RR (same clone as 83131-8-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 83131-8-RR (NLRP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NLRP10 Recombinant antibody (83131-8-RR)

    FC experiment of A431 using 83131-8-RR (same clone as 83131-8-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NLRP10 Recombinant antibody (83131-8-RR)

    FC experiment of HeLa using 83131-8-RR (same clone as 83131-8-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83131-8-RR

    Affinity and Kinetic Characterization of 83131-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83131-8-RR against Human NLRP10 were performed. The affinity constant is 0.899 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83131-8-PBS is NLRP10 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NLRP10 fusion protein Ag26402 相同性解析による交差性が予測される生物種
Full Name NLR family, pyrin domain containing 10
Calculated molecular weight 655 aa, 75 kDa
GenBank accession numberBC104957
Gene symbol NLRP10
Gene ID (NCBI) 338322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.