Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of A549 using 15182-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15182-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 15182-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 15182-1-AP
IP result of anti-NLRP2 (IP:15182-1-AP, 4ug; Detection:15182-1-AP 1:3000) with A549 cells lysate 920 ug.
IHC staining of human placenta using 15182-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15182-1-AP (NLRP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15182-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15182-1-AP (NLRP2 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

NLRP2 Polyclonal antibody

NLRP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15182-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CLR19.9, NALP2, NBS1, NLRP2, PAN1, PYPAF2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, human lung tissue
Positive IP detected in	A549 cells
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

15182-1-AP targets NLRP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NLRP2 fusion protein Ag7167 相同性解析による交差性が予測される生物種
Full Name	NLR family, pyrin domain containing 2
Calculated molecular weight	121 kDa
Observed molecular weight	105-110 kDa
GenBank accession number	BC001039
Gene Symbol	NLRP2
Gene ID (NCBI)	55655
RRID	AB_2298531
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX02
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NLRP2 antibody 15182-1-AP	Download protocol
IHC protocol for NLRP2 antibody 15182-1-AP	Download protocol
IP protocol for NLRP2 antibody 15182-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Front Immunol Macrophage IL-1β contributes to tumorigenesis through paracrine AIM2 inflammasome activation in the tumor microenvironment Authors - Zhi Huan Chew View Article
mouse	WB,IHC	Brain Behav Immun Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression. Authors - Qingyu Zhang KD Validated View Article
human	WB	Eur J Immunol NLRP2 negatively regulates antiviral immunity by interacting with TBK1. Authors - Yanqing Yang KD Validated View Article
human	IHC	Front Microbiol Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4. Authors - Pei-Hui Ding View Article
mouse	IHC	Mol Cell Endocrinol Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes Authors - Mengjun Zhang View Article
mouse	WB	J Neuroimmunol A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease. Authors - Shuxuan Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 15182-1-AP

WB analysis of A549 using 15182-1-AP

WB analysis of human lung using 15182-1-AP

IP experiment of A549 using 15182-1-AP

IHC staining of human placenta using 15182-1-AP

IHC staining of human placenta using 15182-1-AP

NLRP2 Polyclonal antibody

NLRP2 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Macrophage IL-1β contributes to tumorigenesis through paracrine AIM2 inflammasome activation in the tumor microenvironment

Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression.

NLRP2 negatively regulates antiviral immunity by interacting with TBK1.

Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.

Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes

A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease.