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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NLRP3 Monoclonal antibody (68102-1-Ig)

    WB analysis using 68102-1-Ig (same clone as 68102-1-PBS)

    Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NLRP3 Monoclonal antibody (68102-1-Ig)

    WB analysis using 68102-1-Ig (same clone as 68102-1-PBS)

    Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NLRP3 Monoclonal antibody (68102-1-Ig)

    WB analysis using 68102-1-Ig (same clone as 68102-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50203-1

    Sandwich ELISA standard curve of MP50203-1

    Sandwich ELISA standard curve of MP50203-1, Human NLRP3 Monoclonal Matched Antibody Pair - PBS only. 68102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26289. 68102-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP50203-1

    Sample test of MP50203-1

    The mean NLRP3 concentration was determined to be 27.37 ng/mL in THP-1 cell extract based on a 1.50 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NLRP3 Monoclonal antibody (68102-1-Ig)

    IHC staining of human kidney using 68102-1-Ig (same clone as 68102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NLRP3 Monoclonal antibody (68102-1-Ig)

    IHC staining of human kidney using 68102-1-Ig (same clone as 68102-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68102-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50203-1

    Cytometric bead array standard curve of MP50203-1

    Cytometric bead array standard curve of MP50203-1, NLRP3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68102-1-PBS. Detection antibody: 68102-2-PBS. Standard:Ag26289. Range: 1.563-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

NLRP3 Monoclonal antibody, PBS Only (Capture)

NLRP3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68102-1-PBS
Clone No.3H1A7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

3H1A7, Angiotensin/vasopressin receptor AII/AVP-like, C1orf7, CIAS1, Cold-induced autoinflammatory syndrome 1 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68102-1-PBS targets NLRP3 as part of a matched antibody pair:

MP50203-1: 68102-1-PBS capture and 68102-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NLRP3 fusion protein Ag26289 相同性解析による交差性が予測される生物種
Full Name NLR family, pyrin domain containing 3
Calculated molecular weight 118 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_001079821
Gene Symbol NLRP3
Gene ID (NCBI) 114548
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa(PMID: 17164409, 34680443).