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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NLRP7 Recombinant antibody

NLRP7 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241120A10

Cat no : 83996-4-RR

Synonyms

NOD12, NALP7, NACHT, LRR and PYD domains-containing protein 7, HYDM, CLR19.4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using NLRP7 Recombinant antibody (83996-4-RR)

    IF Staining of K-562 using 83996-4-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using NLRP7 antibody (83996-4-RR, Clone: 241120A10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83996-4-RR

    Affinity and Kinetic Characterization of 83996-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83996-4-RR against Human NLRP7 were performed. The affinity constant is 1.98 nM.

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Tested Applications

Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83996-4-RR is NLRP7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NLRP7 fusion protein Ag27465 相同性解析による交差性が予測される生物種
Full Name NLR family, pyrin domain containing 7
Calculated molecular weight112 kDa, 115 kDa, 118 kDa
GenBank accession numberBC109125
Gene symbol NLRP7
Gene ID (NCBI) 199713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NLRP7, also called NALP7 or PYPAF3, is a member of the NOD-like receptors (NLR), a family of proteins that plays a crucial role in the innate immune response. NLRP7 has been reported to emerge from NLRP2 gene. Similar to all inflammasomes, NLRP7 contributes to both pro- and an anti-inflammatory processes, depending on whether it functions in an inflammasome-dependent or independent pathway. The function of NLRP7 inflammasome has also been reported to depend on the master regulatory transcription factor protein that controls cellular inflammation, the NF-kB. In addition to its pro- and anti-inflammatory actions, NLRP7 plays a role in restricting intracellular bacterial replication and can also induce inflammasome assembly upon specific stimulation by FSL-1 (diacylated lipoprotein).

Protocols

Product Specific Protocols
IF protocol for NLRP7 antibody 83996-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols