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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    WB analysis using 27232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of rat brain tissue using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    IP experiment of rat brain using 27232-1-AP

    IP result of anti-NMDAR1/GRIN1 (IP:27232-1-AP, 4ug; Detection:27232-1-AP 1:400) with rat brain tissue lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    IHC staining of mouse brain using 27232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    IHC staining of mouse cerebellum using 27232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    IHC staining of mouse cerebellum using 27232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27232-1-AP (NMDAR1/GRIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    IF Staining of rat brain using 27232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1/GRIN1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NMDAR1/GRIN1 Polyclonal antibody (27232-1-AP)

    IF Staining of rat brain using 27232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1/GRIN1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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NMDAR1/GRIN1 Polyclonal antibody

NMDAR1/GRIN1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 27232-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

GRIN1, NMDAR1, NMD-R1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27232-1-AP targets NMDAR1/GRIN1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMDAR1/GRIN1 fusion protein Ag26093 相同性解析による交差性が予測される生物種
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Calculated molecular weight 105 kDa
Observed molecular weight116-120 kDa
GenBank accession numberNM_000832
Gene Symbol NMDAR1
Gene ID (NCBI) 2902
RRIDAB_3085939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).

Protocols

Product Specific Protocols
WB protocol for NMDAR1/GRIN1 antibody 27232-1-APDownload protocol
IHC protocol for NMDAR1/GRIN1 antibody 27232-1-APDownload protocol
IF protocol for NMDAR1/GRIN1 antibody 27232-1-APDownload protocol
IP protocol for NMDAR1/GRIN1 antibody 27232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols