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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NME1 Recombinant antibody (85038-7-RR)

    WB analysis using 85038-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85038-7-RR (NME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NME1 Recombinant antibody (85038-7-RR)

    WB analysis using 85038-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85038-7-RR (NME1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NME1 Recombinant antibody (85038-7-RR)

    IF Staining of MCF-7 using 85038-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using NME1 antibody (85038-7-RR, Clone: 242670D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using NME1 Recombinant antibody (85038-7-RR)

    FC experiment of HepG2 using 85038-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NME1 Recombinant antibody (85038-7-RR, Clone:242670D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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NME1 Recombinant antibody

NME1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85038-7-RR
Clone No.242670D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Granzyme A-activated DNase, Granzyme A activated DNase, GAAD, EC:2.7.4.6, AWD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, A549 cells, MCF-7 cells, Jurkat cells, C6 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85038-7-RR targets NME1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NME1 fusion protein Ag1548 相同性解析による交差性が予測される生物種
Full Name non-metastatic cells 1, protein (NM23A) expressed in
Calculated molecular weight 17 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC018994
Gene Symbol NME1
Gene ID (NCBI) 4830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15531
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NME1 is also named as NDPKA(Nucleoside diphosphate kinase A), NM23(Metastasis inhibition factor nm23),GAAD(Granzyme A-activated DNase) and belongs to the NDK family.It is a major role in the synthesis of nucleoside triphosphates other than ATP and required for neural development including neural patterning and cell fate determination.It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NME1 antibody 85038-7-RRDownload protocol
IF protocol for NME1 antibody 85038-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols