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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NME2 Recombinant antibody

NME2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242698C7

Cat no : 85074-2-RR

Synonyms

NDKB, Histidine protein kinase NDKB, EC:2.7.4.6, EC:2.7.13.3, C-myc purine-binding transcription factor PUF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NME2 Recombinant antibody (85074-2-RR)

    WB analysis using 85074-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293T cells using NME2 Recombinant antibody (85074-2-RR)

    FC experiment of HEK-293T using 85074-2-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85074-2-RR targets NME2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NME2 fusion protein Ag13913 相同性解析による交差性が予測される生物種
Full Name non-metastatic cells 2, protein (NM23B) expressed in
Calculated molecular weight 152 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC002476
Gene symbol NME2
Gene ID (NCBI) 4831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NME2 antibody 85074-2-RRDownload protocol
FC protocol for NME2 antibody 85074-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols