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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NME2 Recombinant antibody (85074-4-RR)

    WB analysis using 85074-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85074-4-RR (NME2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using NME2 Recombinant antibody (85074-4-RR)

    IF Staining of Jurkat using 85074-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NME2 antibody (85074-4-RR, Clone: 242698C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NME2 Recombinant antibody

NME2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85074-4-RR
Clone No.242698C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NDKB, Histidine protein kinase NDKB, EC:2.7.4.6, EC:2.7.13.3, C-myc purine-binding transcription factor PUF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, U-87 MG cells, OVCAR-3 cells, rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85074-4-RR targets NME2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NME2 fusion protein Ag13913 相同性解析による交差性が予測される生物種
Full Name non-metastatic cells 2, protein (NM23B) expressed in
Calculated molecular weight 152 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC002476
Gene Symbol NME2
Gene ID (NCBI) 4831
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22392
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for NME2 antibody 85074-4-RRDownload protocol
IF protocol for NME2 antibody 85074-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols