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  • Featured Product
  • KD/KO Validated

NME3 Polyclonal antibody

NME3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15136-1-AP

Synonyms

NDP kinase 3, NDK 3, KIAA0516, EC:2.7.4.6, DR-nm23

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NME3 Polyclonal antibody (15136-1-AP)

    WB analysis using 15136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using NME3 Polyclonal antibody (15136-1-AP)

    WB analysis of mouse kidney using 15136-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NME3 Polyclonal antibody (15136-1-AP)

    WB analysis of MCF-7 using 15136-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15136-1-AP (NME3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NME3 Polyclonal antibody (15136-1-AP)

    IP experiment of HEK-293 using 15136-1-AP

    IP result of anti-NME3 (IP:15136-1-AP, 4ug; Detection:15136-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NME3 Polyclonal antibody (15136-1-AP)

    IHC staining of human breast cancer using 15136-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NME3 Polyclonal antibody (15136-1-AP)

    IHC staining of human breast cancer using 15136-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15136-1-AP (NME3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NME3 Polyclonal antibody (15136-1-AP)

    IF Staining of HeLa using 15136-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NME3 antibody (15136-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NME3 Polyclonal antibody (15136-1-AP)

    IF Staining of MCF-7 using 15136-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15136-1-AP (NME3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse kidney tissue, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15136-1-AP targets NME3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NME3 fusion protein Ag7284 相同性解析による交差性が予測される生物種
Full Name non-metastatic cells 3, protein expressed in
Calculated molecular weight 19 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC000250
Gene symbol NME3
Gene ID (NCBI) 4832
RRIDAB_10858322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6).

Protocols

Product Specific Protocols
WB protocol for NME3 antibody 15136-1-APDownload protocol
IHC protocol for NME3 antibody 15136-1-APDownload protocol
IF protocol for NME3 antibody 15136-1-APDownload protocol
IP protocol for NME3 antibody 15136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,zebrafishIF,WB

J Biol Chem

The nucleoside diphosphate kinase NME3 associates with nephronophthisis proteins, and is required for ciliary function during renal development.

Authors - Sylvia Hoff
  • KD Validated
View Article