Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, human kidney tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
12923-1-AP targets NME5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NME5 fusion protein Ag3610 相同性解析による交差性が予測される生物種 |
| Full Name | non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase) |
| Calculated molecular weight | 212 aa, 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC026182 |
| Gene Symbol | NME5 |
| Gene ID (NCBI) | 8382 |
| RRID | AB_2152973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NME5 antibody 12923-1-AP | Download protocol |
| IHC protocol for NME5 antibody 12923-1-AP | Download protocol |
| IP protocol for NME5 antibody 12923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
J Hazard Mater TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis. | ||
Int J Cancer Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma. | ||
Cell Rep Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice | ||
Sci China Life Sci Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes |