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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NME6 Polyclonal antibody

NME6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10250-1-AP

Synonyms

IPIA ALPHA, NDK 6, NDP kinase 6, NME6

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using NME6 Polyclonal antibody (10250-1-AP)

    WB analysis of LNCaP using 10250-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 10250-1-AP (NME6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using NME6 Polyclonal antibody (10250-1-AP)

    WB analysis of U2OS using 10250-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 10250-1-AP (NME6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NME6 Polyclonal antibody (10250-1-AP)

    IHC staining of human colon cancer using 10250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NME6 Polyclonal antibody (10250-1-AP)

    IHC staining of human colon cancer using 10250-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10250-1-AP (NME6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10250-1-AP targets NME6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NME6 fusion protein Ag0315 相同性解析による交差性が予測される生物種
Full Name non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase)
Calculated molecular weight 22 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC001808
Gene symbol NME6
Gene ID (NCBI) 10201
RRIDAB_2298600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NME6 antibody 10250-1-APDownload protocol
IHC protocol for NME6 antibody 10250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols