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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NMI Recombinant antibody, PBS Only

NMI Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241135H2

Cat no : 83986-5-PBS

Synonyms

N-myc-interactor, N-myc and STAT interactor, N myc interactor, N myc and STAT interactor, N myc (and STAT) interactor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NMI Recombinant antibody (83986-5-RR)

    WB analysis using 83986-5-RR (same clone as 83986-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83986-5-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83986-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NMI Recombinant antibody (83986-5-RR)

    FC experiment of HepG2 using 83986-5-RR (same clone as 83986-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-5-RR, Clone:241135H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83986-5-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83986-5-PBS is NMI Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NMI fusion protein Ag34759 相同性解析による交差性が予測される生物種
Full Name N-myc (and STAT) interactor
Calculated molecular weight 35 kDa
Observed molecular weight37 kDa
GenBank accession numberBC001268
Gene symbol NMI
Gene ID (NCBI) 9111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.