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KD/KO Validated

NMT1 Polyclonal antibody

Name: NMT1 Polyclonal antibody
Brand: Proteintech
SKU: 11546-1-AP

NMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11546-1-AP

Synonyms

N myristoyltransferase 1, Myristoyl-CoA:protein N-myristoyltransferase 1, HsNMT1, Glycylpeptide N-tetradecanoyltransferase 1, EC:2.3.1.97

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of SKOV-3 using 11546-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11546-1-AP
WB result of NMT1 antibody (11546-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected HeLa cells.
WB analysis of PC-3 using 11546-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11546-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11546-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11546-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11546-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11546-1-AP
IP result of anti-NMT1 (IP:11546-1-AP, 4ug; Detection:11546-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human gliomas using 11546-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11546-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11546-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 11546-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 11546-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 11546-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NMT1 antibody (11546-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 11546-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SKOV-3 cells, PC-3 cells, mouse pancreas tissue, HeLa cells, L02 cells, human kidney tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human gliomas tissue, human heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

11546-1-AP targets NMT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NMT1 fusion protein Ag2072 相同性解析による交差性が予測される生物種
Full Name	N-myristoyltransferase 1
Calculated molecular weight	496 aa, 57 kDa
Observed molecular weight	49-68 kDa
GenBank accession number	BC006569
Gene symbol	NMT1
Gene ID (NCBI)	4836
RRID	AB_2153157
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.

Protocols

Product Specific Protocols
WB protocol for NMT1 antibody 11546-1-AP	Download protocol
IHC protocol for NMT1 antibody 11546-1-AP	Download protocol
IF protocol for NMT1 antibody 11546-1-AP	Download protocol
IP protocol for NMT1 antibody 11546-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. Authors - Janja Božič View Article
human	WB,IF	Cancer Lett N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression. Authors - Yi Sun KD Validated View Article
human	WB,IHC	Cell Death Dis NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway. Authors - Lu Deng KD Validated View Article
human	WB	FASEB J Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases. Authors - Perinpanayagam Maneka A MA View Article
mouse	WB	Biochem Biophys Res Commun N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum. Authors - Elzbieta Dudek View Article
human		Oncotarget Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model. Authors - Begoña Díaz View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NMT1 Polyclonal antibody

NMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of SKOV-3 using 11546-1-AP

WB analysis of HeLa using 11546-1-AP

WB analysis of PC-3 using 11546-1-AP

WB analysis of human kidney using 11546-1-AP

WB analysis of mouse pancreas using 11546-1-AP

WB analysis of HeLa using 11546-1-AP

WB analysis of L02 using 11546-1-AP

IP experiment of HeLa using 11546-1-AP

IHC staining of human gliomas using 11546-1-AP

IHC staining of human kidney using 11546-1-AP

IHC staining of human kidney using 11546-1-AP

IHC staining of human heart using 11546-1-AP

IHC staining of human heart using 11546-1-AP

IF Staining of MCF-7 using 11546-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression.

NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway.

Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases.

N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum.

Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.