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NMT1 Monoclonal antibody

NMT1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D9B9

Cat no : 67984-1-Ig

Synonyms

N myristoyltransferase 1, NMT1, Type I N myristoyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NMT1 Monoclonal antibody (67984-1-Ig)

    WB analysis using 67984-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67984-1-Ig (NMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of SKOV-3 cells using NMT1 Monoclonal antibody (67984-1-Ig)

    WB analysis of SKOV-3 using 67984-1-Ig

    WB result of NMT1 antibody (67984-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected SKOV-3 cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NMT1 Monoclonal antibody (67984-1-Ig)

    IHC staining of human breast cancer using 67984-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NMT1 Monoclonal antibody (67984-1-Ig)

    IHC staining of human liver cancer using 67984-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NMT1 Monoclonal antibody (67984-1-Ig)

    IHC staining of human liver cancer using 67984-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67984-1-Ig (NMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, L02 cells, PC-3 cells, SKOV-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67984-1-Ig targets NMT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NMT1 fusion protein Ag20541 相同性解析による交差性が予測される生物種
Full Name N-myristoyltransferase 1
Calculated molecular weight 496 aa, 57 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC006569
Gene symbol NMT1
Gene ID (NCBI) 4836
RRIDAB_2918733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.

Protocols

Product Specific Protocols
WB protocol for NMT1 antibody 67984-1-IgDownload protocol
IHC protocol for NMT1 antibody 67984-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols