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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NMT2 Polyclonal antibody

NMT2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15720-1-AP

Synonyms

Peptide N-myristoyltransferase 2, N myristoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Glycylpeptide N-tetradecanoyltransferase 2, EC:2.3.1.97

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using NMT2 Polyclonal antibody (15720-1-AP)

    IHC staining of mouse testis using 15720-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15720-1-AP (NMT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NMT2 Polyclonal antibody (15720-1-AP)

    IF Staining of U2OS using 15720-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NMT2 antibody (15720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15720-1-AP targets NMT2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NMT2 fusion protein Ag8438 相同性解析による交差性が予測される生物種
Full Name N-myristoyltransferase 2
Calculated molecular weight529aa,60 kDa; 498aa,57 kDa
GenBank accession numberBC006376
Gene symbol NMT2
Gene ID (NCBI) 9397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for NMT2 antibody 15720-1-APDownload protocol
IF protocol for NMT2 antibody 15720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols