mouse liver tissue were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

L02 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

HepG2 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HepG2 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

L02 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP result of anti-NNMT (IP:15123-1-AP, 4ug; Detection:15123-1-AP 1:300) with HepG2 cells lysate 2400ug.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15123-1-AP (NNMT antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15123-1-AP (NNMT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NNMT (15123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).