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NNMT Polyclonal antibody

Name: NNMT Polyclonal antibody
Brand: Proteintech
SKU: 15123-1-AP
Rating: 4.50 (2 reviews)

NNMT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 15123-1-AP

Synonyms

Nicotinamide N-methyltransferase, EC:2.1.1.1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of mouse liver using 15123-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 15123-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15123-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15123-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 15123-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 15123-1-AP (NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15123-1-AP
IP result of anti-NNMT (IP:15123-1-AP, 4ug; Detection:15123-1-AP 1:300) with HepG2 cells lysate 2400ug.
IHC staining of human stomach cancer using 15123-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15123-1-AP (NNMT antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15123-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15123-1-AP (NNMT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 15123-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NNMT (15123-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 15123-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	L02 cells, HepG2 cells, mouse liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 18 publications below
IHC	See 6 publications below
IF	See 2 publications below

Product Information

15123-1-AP targets NNMT in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NNMT fusion protein Ag7197 相同性解析による交差性が予測される生物種
Full Name	nicotinamide N-methyltransferase
Calculated molecular weight	30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC000234
Gene symbol	NNMT
Gene ID (NCBI)	4837
RRID	AB_2153579
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NNMT antibody 15123-1-AP	Download protocol
IHC protocol for NNMT antibody 15123-1-AP	Download protocol
IF protocol for NNMT antibody 15123-1-AP	Download protocol
IP protocol for NNMT antibody 15123-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Rep Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis Authors - Jia Yan View Article
human	WB,IHC,IF	Oncogene Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids Authors - Hui Zhao View Article
mouse	WB	Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. Authors - Ferrol I Rome View Article
human	WB	Mol Oncol Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform. Authors - Jie Li KD Validated View Article
human	WB	Front Cell Infect Microbiol Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells. Authors - Fengxue Geng View Article
human	IHC	Sci Rep The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease. Authors - Rina Takahashi View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Kenzo (Verified Customer) (01-05-2024)	This antibody worked for immunofluorescence with a little background Applications: Immunofluorescence Primary Antibody Dilution: 1:10000 Cell Tissue Type: Mouse brain
FH Johnny (Verified Customer) (01-07-2022)	products from proteintech have always been reliable. Applications: Western Blot

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NNMT Polyclonal antibody

NNMT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of mouse liver using 15123-1-AP

WB analysis using 15123-1-AP

WB analysis of HepG2 using 15123-1-AP

WB analysis of HepG2 using 15123-1-AP

WB analysis of L02 using 15123-1-AP

IP experiment of HepG2 using 15123-1-AP

IHC staining of human stomach cancer using 15123-1-AP

IF Staining of HepG2 using 15123-1-AP

FC experiment of HepG2 using 15123-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis

Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids

Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.

Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.

Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.

The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease.

Reviews

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