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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NNMT Recombinant antibody (84876-5-RR)

    WB analysis using 84876-5-RR (same clone as 84876-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84876-5-RR (NNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84876-5-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using NNMT Recombinant antibody (84876-5-RR)

    IP experiment of L02 using 84876-5-RR (same clone as 84876-5-PBS)

    IP result of anti-NNMT (IP:84876-5-RR, 4ug; Detection:84876-5-RR 1:1500) with L02 cells lysate 1120 ug. This data was developed using the same antibody clone with 84876-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NNMT Recombinant antibody (84876-5-RR)

    FC experiment of HepG2 using 84876-5-RR (same clone as 84876-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NNMT Recombinant antibody (84876-5-RR, Clone:242394B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84876-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84876-5-RR

    Affinity and Kinetic Characterization of 84876-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84876-5-RR against Human NNMT were performed. The affinity constant is 0.824 nM.

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NNMT Recombinant antibody, PBS Only

NNMT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 84876-5-PBS
Clone No.242394B7

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

Nicotinamide N-methyltransferase, EC:2.1.1.1, 242394B7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84876-5-PBS targets NNMT in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NNMT fusion protein Ag7197 相同性解析による交差性が予測される生物種
Full Name nicotinamide N-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000234
Gene Symbol NNMT
Gene ID (NCBI) 4837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40261
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091).