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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using NNT Monoclonal antibody (68194-1-Ig)

    WB analysis of LNCaP using 68194-1-Ig (same clone as 68194-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NNT Monoclonal antibody (68194-1-Ig)

    WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NNT Monoclonal antibody (68194-1-Ig)

    WB analysis using 68194-1-Ig (same clone as 68194-1-PBS)

    Various lysates (mouse heart tissue and mouse liver tissue were dissected from BALB/c mice) were subjected to SDS PAGE followed by western blot with 68194-1-Ig (NNT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NNT Monoclonal antibody (68194-1-Ig)

    IP experiment of HepG2 using 68194-1-Ig (same clone as 68194-1-PBS)

    IP result of anti-NNT (IP:68194-1-Ig, 4ug; Detection:68194-1-Ig 1:5000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NNT Monoclonal antibody (68194-1-Ig)

    IHC staining of mouse heart using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NNT Monoclonal antibody (68194-1-Ig)

    IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using NNT Monoclonal antibody (68194-1-Ig)

    IHC staining of human liver using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NNT Monoclonal antibody (68194-1-Ig)

    IHC staining of mouse heart using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68194-1-Ig (NNT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NNT Monoclonal antibody (68194-1-Ig)

    IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NNT Monoclonal antibody (68194-1-Ig)

    IF Staining of HeLa using 68194-1-Ig (same clone as 68194-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NNT antibody (68194-1-Ig, Clone: 1F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68194-1-PBS in a different storage buffer formulation.

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NNT Monoclonal antibody, PBS Only

NNT Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 68194-1-PBS
Clone No.1F5H6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

NNT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68194-1-PBS targets NNT in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NNT fusion protein Ag4259 相同性解析による交差性が予測される生物種
Full Name nicotinamide nucleotide transhydrogenase
Calculated molecular weight 1085 aa, 114 kDa
Observed molecular weight114 kDa
GenBank accession numberBC032370
Gene Symbol NNT
Gene ID (NCBI) 23530
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13423
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and β subunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 114 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).