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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using NOB1 Polyclonal antibody (14456-1-AP)

    WB analysis of Jurkat using 14456-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14456-1-AP (NOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NOB1 Polyclonal antibody (14456-1-AP)

    WB analysis of L02 using 14456-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14456-1-AP (NOB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using NOB1 Polyclonal antibody (14456-1-AP)

    FC experiment of Jurkat using 14456-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug NOB1 Polyclonal antibody (14456-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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NOB1 Polyclonal antibody

NOB1 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 14456-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

NOB1P, MSTP158, MST158, EC:3.1.-.-, ART4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, L02 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14456-1-AP targets NOB1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOB1 fusion protein Ag6051 相同性解析による交差性が予測される生物種
Full Name NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Calculated molecular weight 46 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC064630
Gene Symbol NOB1
Gene ID (NCBI) 28987
RRIDAB_2236130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULX3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for NOB1 antibody 14456-1-APDownload protocol
FC protocol for NOB1 antibody 14456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Am J Transl Res

miRNA-612 suppresses ovarian cancer cell tumorigenicity by downregulating NOB1.

Authors - Zhikun Shi
View Article