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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using NOB1 Monoclonal antibody (66048-1-Ig)

    WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using NOB1 Monoclonal antibody (66048-1-Ig)

    WB analysis of human placenta using 66048-1-Ig (same clone as 66048-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOB1 Monoclonal antibody (66048-1-Ig)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOB1 Monoclonal antibody (66048-1-Ig)

    IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.

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NOB1 Monoclonal antibody, PBS Only

NOB1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66048-1-PBS
Clone No.8C11A12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66048-1-PBS targets NOB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOB1 fusion protein Ag17907 相同性解析による交差性が予測される生物種
Full Name NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC000050
Gene Symbol NOB1
Gene ID (NCBI) 28987
RRIDAB_11042447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9ULX3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.