Validation Data Gallery
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Tested Applications
| Positive WB detected in | human liver tissue, L02 cells |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
15297-1-AP targets RPH3AL in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPH3AL fusion protein Ag7552 相同性解析による交差性が予測される生物種 |
| Full Name | rabphilin 3A-like (without C2 domains) |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC005153 |
| Gene Symbol | RPH3AL |
| Gene ID (NCBI) | 9501 |
| RRID | AB_2181270 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNE2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPH3AL antibody 15297-1-AP | Download protocol |
| IP protocol for RPH3AL antibody 15297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2. | ||
Clin Chim Acta Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer. |