Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, THP-1 cells, K-562 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
66710-1-Ig targets NOD2 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | nucleotide-binding oligomerization domain containing 2 |
| Calculated molecular weight | 115 kDa |
| GenBank accession number | NM_022162 |
| Gene Symbol | NOD2 |
| Gene ID (NCBI) | 64127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HC29 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
OD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOD2 antibody 66710-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol CircEpc1 Promotes Ricin Toxin-Induced Inflammation via Activation of NF-κB and MAPK Signaling Pathways by Sponging miR-5114. | ||
Front Cell Infect Microbiol Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways. | ||
J Cell Mol Med β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury. | ||
Front Pharmacol Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells |