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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Noggin Polyclonal antibody

Noggin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30023-1-AP

Synonyms

NOG, noggin, SYM1, SYNS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Noggin Polyclonal antibody (30023-1-AP)

    WB analysis using 30023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30023-1-AP (NOG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-3 cells using Noggin Polyclonal antibody (30023-1-AP)

    FC experiment of PC-3 using 30023-1-AP

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug Noggin Polyclonal antibody (30023-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30023-1-AP targets Noggin in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Noggin fusion protein Ag32755 相同性解析による交差性が予測される生物種
Full Name noggin
Calculated molecular weight 26 kDa
Observed molecular weight 32 kDa, 64 kDa
GenBank accession numberBC034027
Gene symbol Noggin
Gene ID (NCBI) 9241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

Protocols

Product Specific Protocols
WB protocol for Noggin antibody 30023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols