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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOL11 Polyclonal antibody

NOL11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17613-1-AP

Synonyms

L14, NOL11, nucleolar protein 11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOL11 Polyclonal antibody (17613-1-AP)

    WB analysis using 17613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17613-1-AP (NOL11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17613-1-AP targets NOL11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL11 fusion protein Ag11783 相同性解析による交差性が予測される生物種
Full Name nucleolar protein 11
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight75-81 kDa
GenBank accession numberBC064404
Gene symbol NOL11
Gene ID (NCBI) 25926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.

Protocols

Product Specific Protocols
WB protocol for NOL11 antibody 17613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols