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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOL11 Recombinant antibody

NOL11 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240371B5

Cat no : 83391-6-RR

Synonyms

Nucleolar protein 11, L14, 240371B5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOL11 Recombinant antibody (83391-6-RR)

    WB analysis using 83391-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83391-6-RR (NOL11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOL11 Recombinant antibody (83391-6-RR)

    IF Staining of HeLa using 83391-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOL11 Recombinant antibody (83391-6-RR)

    IF Staining of HeLa using 83391-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using NOL11 Recombinant antibody (83391-6-RR)

    FC experiment of HeLa using 83391-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOL11 Recombinant antibody (83391-6-RR, Clone:240371B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83391-6-RR

    Affinity and Kinetic Characterization of 83391-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83391-6-RR against Human NOL11 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HT-29 cells, SW480 cells, A431 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83391-6-RR is NOL11 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NOL11 fusion protein Ag11783 相同性解析による交差性が予測される生物種
Full Name nucleolar protein 11
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight81 kDa
GenBank accession numberBC064404
Gene symbol NOL11
Gene ID (NCBI) 25926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.

Protocols

Product Specific Protocols
WB protocol for NOL11 antibody 83391-6-RRDownload protocol
IF protocol for NOL11 antibody 83391-6-RRDownload protocol
FC protocol for NOL11 antibody 83391-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols