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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOL3 Polyclonal antibody, PBS Only

NOL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10846-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NOL3 Polyclonal antibody (10846-2-AP)

    WB analysis of mouse skeletal muscle using 10846-2-AP (same clone as 10846-2-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NOL3 Polyclonal antibody (10846-2-AP)

    WB analysis of mouse skeletal muscle using 10846-2-AP (same clone as 10846-2-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NOL3 Polyclonal antibody (10846-2-AP)

    WB analysis of HeLa using 10846-2-AP (same clone as 10846-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOL3 Polyclonal antibody (10846-2-AP)

    IHC staining of human breast cancer using 10846-2-AP (same clone as 10846-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NOL3 Polyclonal antibody (10846-2-AP)

    IHC staining of human breast cancer using 10846-2-AP (same clone as 10846-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOL3 Polyclonal antibody (10846-2-AP)

    IF Staining of HepG2 using 10846-2-AP (same clone as 10846-2-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10846-2-AP (NOL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOL3 Polyclonal antibody (10846-2-AP)

    IF Staining of HepG2 using 10846-2-AP (same clone as 10846-2-PBS)

    Immunofluorescent analysis of HepG2 cells, using NOL3 antibody 10846-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10846-2-PBS targets NOL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL3 fusion protein Ag1306 相同性解析による交差性が予測される生物種
Full Name nucleolar protein 3 (apoptosis repressor with CARD domain)
Calculated molecular weight 24 aa, 3 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC012798
Gene symbol NOL3
Gene ID (NCBI) 8996
RRIDAB_2151850
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.