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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOL6 Polyclonal antibody

NOL6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15706-1-AP

Synonyms

NOL6, NRAP, Nucleolar protein 6, UTP22

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SGC-7901 cells using NOL6 Polyclonal antibody (15706-1-AP)

    WB analysis of SGC-7901 using 15706-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NOL6 Polyclonal antibody (15706-1-AP)

    WB analysis of HeLa using 15706-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of PC-3 cells using NOL6 Polyclonal antibody (15706-1-AP)

    WB analysis of PC-3 using 15706-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of SGC-7901 cells using NOL6 Polyclonal antibody (15706-1-AP)

    WB analysis of SGC-7901 using 15706-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

Positive WB detected inSGC-7901 cells, HeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15706-1-AP targets NOL6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL6 fusion protein Ag8378 相同性解析による交差性が予測される生物種
Full Name nucleolar protein family 6 (RNA-associated)
Calculated molecular weight 1146 aa, 128 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC008298
Gene symbol NOL6
Gene ID (NCBI) 65083
RRIDAB_2878169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOL6 antibody 15706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cytotechnology

NOL6, a new founding oncogene in human prostate cancer and targeted by miR-590-3p.

Authors - Degang Dong
View Article