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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HEK-293 using 16083-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HEK-293 using 16083-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HEK-293 using 16083-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HepG2 using 16083-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HepG2 using 16083-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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NOL9 Polyclonal antibody

NOL9 Polyclonal Antibody for WB, ELISA
Cat No. 16083-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA, FRAP

Synonyms

NOL9, nucleolar protein 9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16083-1-AP targets NOL9 in WB, IF, ELISA, FRAP applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL9 fusion protein Ag9082 相同性解析による交差性が予測される生物種
Full Name nucleolar protein 9
Calculated molecular weight 702 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC009257
Gene Symbol NOL9
Gene ID (NCBI) 79707
RRIDAB_11124314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SY16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOL9, also named as Polynucleotide 5'-hydroxyl-kinase NOL9, is a 702 amino acid protein, which belongs to the Clp1 family. NOL9 localizes in the nucleus and as a polynucleotide 5'-kinase is involved in rRNA processing. its polynucleotide kinase activity is required for efficient generation of the 5.8S and 28S rRNAs from the 32S precursor.

Protocols

Product Specific Protocols
WB protocol for NOL9 antibody 16083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFRAP

Nucleic Acids Res

Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity.

Authors - Witold Szaflarski
View Article
humanWB,IF

Mol Biol Cell

LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.

Authors - Castle Christopher D CD
View Article
humanWB

Cancer Res Commun

USP36 SUMOylates Las1L and promotes its function in pre-rRNA ITS2 processing

Authors - Yanping Li
View Article