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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOM1 Polyclonal antibody (29798-1-AP)

    WB analysis using 29798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29798-1-AP (NOM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NOM1 Polyclonal antibody (29798-1-AP)

    IHC staining of mouse cerebellum using 29798-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NOM1 Polyclonal antibody (29798-1-AP)

    IHC staining of mouse cerebellum using 29798-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NOM1 Polyclonal antibody

NOM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29798-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

C7orf3, FLJ16401, NOM1, SGD1, SGD1 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, SW 1990 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29798-1-AP targets NOM1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOM1 fusion protein Ag31423 相同性解析による交差性が予測される生物種
Full Name nucleolar protein with MIF4G domain 1
Calculated molecular weight96KD
Observed molecular weight96~100 kDa
GenBank accession numberNM_138400
Gene Symbol NOM1
Gene ID (NCBI) 64434
RRIDAB_3086164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5C9Z4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleolar protein NOM1, which contains an MIF4G domain and an MA3 domain, was first isolated from the bone marrow of children with acute myeloid leukemia. NOM1 is highly conserved in a variety of species, including in yeast and in humans.

Protocols

Product Specific Protocols
WB protocol for NOM1 antibody 29798-1-APDownload protocol
IHC protocol for NOM1 antibody 29798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols