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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using NOMO2 Polyclonal antibody (14328-1-AP)

    WB analysis of BxPC-3 using 14328-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14328-1-AP (NOMO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NOMO2 Polyclonal antibody (14328-1-AP)

    WB analysis of HEK-293 using 14328-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14328-1-AP (NOMO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using NOMO2 Polyclonal antibody (14328-1-AP)

    IP experiment of HEK-293 using 14328-1-AP

    IP result of anti-NOMO2 (IP:14328-1-AP, 4ug; Detection:14328-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NOMO2 Polyclonal antibody (14328-1-AP)

    IHC staining of human pancreas cancer using 14328-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14328-1-AP (NOMO2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NOMO2 Polyclonal antibody (14328-1-AP)

    IHC staining of human pancreas cancer using 14328-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14328-1-AP (NOMO2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using NOMO2 Polyclonal antibody (14328-1-AP)

    IF Staining of BxPC-3 using 14328-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 14328-1-AP (NOMO2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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NOMO2 Polyclonal antibody

NOMO2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14328-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

pM5 protein 2, Nodal modulator 2, BOS complex subunit NOMO2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14328-1-AP targets NOMO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOMO2 fusion protein Ag5595 相同性解析による交差性が予測される生物種
Full Name NODAL modulator 2
Calculated molecular weight 139 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC041131
Gene Symbol NOMO2
Gene ID (NCBI) 283820
RRIDAB_2152154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JPE7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOMO2 antibody 14328-1-APDownload protocol
IHC protocol for NOMO2 antibody 14328-1-APDownload protocol
IF protocol for NOMO2 antibody 14328-1-APDownload protocol
IP protocol for NOMO2 antibody 14328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Ther

Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.

Authors - Hong Shik Yun
View Article