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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NONO Monoclonal antibody, PBS Only

NONO Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2B10

Cat no : 66361-1-PBS

Synonyms

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of C2C12 cells using NONO Monoclonal antibody (66361-1-Ig)

    WB analysis of C2C12 using 66361-1-Ig (same clone as 66361-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66361-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using NONO Monoclonal antibody (66361-1-Ig)

    WB analysis of ROS1728 using 66361-1-Ig (same clone as 66361-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66361-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NONO Monoclonal antibody (66361-1-Ig)

    WB analysis of mouse skeletal muscle using 66361-1-Ig (same clone as 66361-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66361-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66361-1-PBS targets NONO in WB, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NONO fusion protein Ag17609 相同性解析による交差性が予測される生物種
Full Name non-POU domain containing, octamer-binding
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC010049
Gene symbol NONO
Gene ID (NCBI) 4841
RRIDAB_2881741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.