NOP2 Polyclonal antibody
NOP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10448-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells, C6 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
10448-1-AP targets NOP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NOP2 fusion protein Ag0498 相同性解析による交差性が予測される生物種 |
| Full Name | NOP2 nucleolar protein homolog (yeast) |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 100-120 kDa |
| GenBank accession number | BC000656 |
| Gene symbol | NOP2 |
| Gene ID (NCBI) | 4839 |
| RRID | AB_2282772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13 (PMID: 23775790).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOP2 antibody 10448-1-AP | Download protocol |
| IHC protocol for NOP2 antibody 10448-1-AP | Download protocol |
| IF protocol for NOP2 antibody 10448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Biol Cell RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits. | ||
Transl Cancer Res Effect of NOP2 knockdown on colon cancer cell proliferation, migration, and invasion. | ||
iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges | ||
Cell Mol Life Sci N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst |
