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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOP58 Recombinant antibody

NOP58 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230058C7

Cat no : 82879-1-RR

Synonyms

Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOP58 Recombinant antibody (82879-1-RR)

    WB analysis using 82879-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NOP58 Recombinant antibody (82879-1-RR)

    IP experiment of HeLa using 82879-1-RR

    IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NOP58 Recombinant antibody (82879-1-RR)

    IHC staining of human prostate cancer using 82879-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NOP58 Recombinant antibody (82879-1-RR)

    IHC staining of human prostate cancer using 82879-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NOP58 Recombinant antibody (82879-1-RR)

    IF Staining of U2OS using 82879-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Flow cytometry (FC) experiment of HeLa cells using NOP58 Recombinant antibody (82879-1-RR)

    FC experiment of HeLa using 82879-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOP58 Recombinant antibody (82879-1-RR, Clone:230058C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82879-1-RR

    Affinity and Kinetic Characterization of 82879-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82879-1-RR targets NOP58 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NOP58 fusion protein Ag5740 相同性解析による交差性が予測される生物種
Full Name NOP58 ribonucleoprotein homolog (yeast)
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032592
Gene symbol NOP58
Gene ID (NCBI) 51602
RRIDAB_3670598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

Protocols

Product Specific Protocols
WB protocol for NOP58 antibody 82879-1-RRDownload protocol
IHC protocol for NOP58 antibody 82879-1-RRDownload protocol
IF protocol for NOP58 antibody 82879-1-RRDownload protocol
IP protocol for NOP58 antibody 82879-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols