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  • Featured Product
  • KD/KO Validated

NOSIP Polyclonal antibody

NOSIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27979-1-AP

Synonyms

CGI 25, eNOS interacting protein, NOSIP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOSIP Polyclonal antibody (27979-1-AP)

    WB analysis using 27979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NOSIP Polyclonal antibody (27979-1-AP)

    WB analysis using 27979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NOSIP Polyclonal antibody (27979-1-AP)

    WB analysis of A431 using 27979-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human colon using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human colon using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human lung using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human lung using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOSIP Polyclonal antibody (27979-1-AP)

    IF Staining of HeLa using 27979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27979-1-AP (NOSIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293T cells, A431 cells, A549 cells, Ramos cells, HepG2 cells, L02 cells
Positive IHC detected inhuman colon tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27979-1-AP targets NOSIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOSIP fusion protein Ag27569 相同性解析による交差性が予測される生物種
Full Name nitric oxide synthase interacting protein
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010077
Gene symbol NOSIP
Gene ID (NCBI) 51070
RRIDAB_2881027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOSIP antibody 27979-1-APDownload protocol
IHC protocol for NOSIP antibody 27979-1-APDownload protocol
IF protocol for NOSIP antibody 27979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

iScience

Nosip is a potential therapeutic target in hepatocellular carcinoma cells

Authors - Junjie Gao
  • KD Validated
View Article