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Validation Data Gallery

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  • Western Blot (WB) analysis of hTERT-RPE1 cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    WB analysis of hTERT-RPE1 using 68212-1-Ig (same clone as 68212-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    WB analysis of U-87 MG using 68212-1-Ig (same clone as 68212-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    IF Staining of HeLa using 68212-1-Ig (same clone as 68212-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOTCH2/NOTCH2NL Monoclonal antibody (68212-1-Ig)

    IF Staining of HeLa using 68212-1-Ig (same clone as 68212-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

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NOTCH2/NOTCH2NL Monoclonal antibody, PBS Only

NOTCH2/NOTCH2NL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68212-1-PBS
Clone No.2D5E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68212-1-PBS targets NOTCH2/NOTCH2NL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOTCH2/NOTCH2NL fusion protein Ag1679 相同性解析による交差性が予測される生物種
Full Name Notch homolog 2 (Drosophila) N-terminal like
Calculated molecular weight 265 kDa
Observed molecular weight250-265 kDa
GenBank accession numberBC019835
Gene Symbol NOTCH2NL
Gene ID (NCBI) 388677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP0DPK4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Notch 2 is a member of the notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch2, like other Norch genes, functions in development of various tissues. This antibody can recognize NOTCH2 and NOTCH2NL. The antibody detects the full-length NOTCH2 protein about 250-265 kDa.