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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis using 66956-1-Ig (same clone as 66956-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis of HepG2 using 66956-1-Ig (same clone as 66956-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis of MCF-7 using 66956-1-Ig (same clone as 66956-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using NOTUM Monoclonal antibody (66956-1-Ig)

    WB analysis of T-47D using 66956-1-Ig (same clone as 66956-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NOTUM Monoclonal antibody (66956-1-Ig)

    IHC staining of human small intestine using 66956-1-Ig (same clone as 66956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 10x lens).. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using NOTUM Monoclonal antibody (66956-1-Ig)

    IHC staining of human small intestine using 66956-1-Ig (same clone as 66956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 40x lens).. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50968-3

    Cytometric bead array standard curve of MP50968-3

    Cytometric bead array standard curve of MP50968-3, NOTUM Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66956-2-PBS. Detection antibody: 66956-1-PBS. Standard:Ag6338. Range: 0.098-100 ng/mL.

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NOTUM Monoclonal antibody, PBS Only (Detector)

NOTUM Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66956-1-PBS
Clone No.2F6D5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

Protein notum homolog, Palmitoleoyl-protein carboxylesterase NOTUM, OK/SW-CL.30, hNOTUM, EC:3.1.1.98

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66956-1-PBS targets NOTUM as part of a matched antibody pair:

MP50968-3: 66956-2-PBS capture and 66956-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NOTUM fusion protein Ag6338 相同性解析による交差性が予測される生物種
Full Name notum pectinacetylesterase homolog (Drosophila)
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC060882
Gene Symbol NOTUM
Gene ID (NCBI) 147111
RRIDAB_2882279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P988
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.