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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NOV Polyclonal antibody

NOV Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55449-1-AP

Synonyms

CCN family member 3, CCN3, IBP 9, IGFBP 9, IGFBP9, NOV

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CCN3 Polyclonal antibody (55449-1-AP)

    WB analysis of mouse liver using 55449-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55449-1-AP (NOV antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human nephroblastoma tissue using CCN3 Polyclonal antibody (55449-1-AP)

    IHC staining of human nephroblastoma using 55449-1-AP

    Immunohistochemical analysis of paraffin-embedded human nephroblastoma using 55449-1-AP (NOV antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CCN3 Polyclonal antibody (55449-1-AP)

    IHC staining of human prostate cancer using 55449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 55449-1-AP (NOV antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CCN3 Polyclonal antibody (55449-1-AP)

    IHC staining of human prostate cancer using 55449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 55449-1-AP (NOV antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive IHC detected inhuman nephroblastoma tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

55449-1-AP targets NOV in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nephroblastoma overexpressed gene
Calculated molecular weight 39 kDa
GenBank accession numberNM_002514
Gene symbol NOV
Gene ID (NCBI) 4856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOV antibody 55449-1-APDownload protocol
IHC protocol for NOV antibody 55449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cell Mol Med

NOV/CCN3 induces cartilage protection by inhibiting PI3K/AKT/mTOR pathway.

Authors - Xiaojian Huang
View Article